Summary

Patients with cell-mediated immunodeficiency experience recurrent infections with a broad range of pathogens, and an accompanying humoral immunodeficiency is not uncommon. A persistently low lymphocyte differential on a full blood count may provide a clue and should prompt further testing with quantification of lymphocyte subsets. Measurement of total immunoglobulins is a first-line screening investigation in suspected humoral immunodeficiency. Further investigations, which provide an in vitro or in vivo functional assessment, are highly specialised assays which are difficult to perform and interpret. Consultation with a specialist immunologist and the diagnostic laboratory is recommended.

Introduction

Defence against potentially harmful pathogens is achieved by physical barriers such as skin and mucous membranes, and the coordinated efforts of the innate and adaptive immune systems. Innate immune responses are carried out by macrophages, neutrophils and natural killer cells, together with cytokines, complement and acute phase reactants such as C-reactive protein. Adaptive immunity relies upon B and T lymphocytes which express antigen-specific surface receptors. It can be divided into humoral (antibody-mediated and dependent upon B lymphocytes) and cellular (coordinated by T lymphocytes) immunity. While this distinction is oversimplified and somewhat inaccurate in that both types of responses are dependent upon helper T lymphocytes, it provides a useful model for classifying and evaluating suspected immunodeficiency.

Immunodeficiency

This occurs when failure of any part of the immune system leads to an increased predisposition to infection and associated sequelae such as autoimmunity and malignancy. Primary immunodeficiency results from genetic mutations of components intrinsic to the immune system. Clinical diagnosis should be accompanied by molecular identification of a genetic mutation wherever possible to confirm the diagnosis, identify genotype-phenotype correlation, assist with genetic counselling and identify suitable candidates for gene-specific therapy. Secondary immunodeficiency results from defective immune function as a consequence of another condition such as HIV infection. Drugs such as corticosteroids, azathioprine, methotrexate or cyclosporin can also cause secondary immunodeficiency. Subtle impairment of immune function can also accompany certain chronic medical conditions including diabetes and chronic renal failure.

Immunodeficiency can be classified functionally into humoral or cell-mediated arms, as dysfunction of either pathway is characterised by specific clinical presentations (Table 1). Possible investigations for suspected immunodeficiency are presented in Table 2. These tests should be performed when the patient is clinically well, and not during an acute infective illness.

Table 1 Characteristic clinical presentations of immunodeficiency
Type of immunodeficiency
Clinical presentation
Humoral immunodeficiency
Hypogammaglobulinaemia
Recurrent sinopulmonary infection:
- Streptococcus pneumoniae
- Haemophilus influenzae
- Neisseria species
Other bacterial infections such as gastrointestinal, central nervous system, joint
Evidence of end-organ damage such as bronchiectasis, conductive hearing loss
Cellular immunodeficiency
T cell dysfunction
Infections with:
- intracellular bacteria (mycobacteria, salmonella)
- viruses (Epstein Barr, cytomegalovirus, varicella zoster,
herpes simplex)
- fungi (candida, aspergillus, cryptococcus, histoplasma,
pneumocystis)
- protozoa (toxoplasma, microsporidium, cryptosporidium)
Interleukin-12 interferon gamma axis deficiency
Atypical mycobacterial and salmonella infections
Impaired response to Candida species
Persistent mucocutaneous candidiasis
Autoimmune endocrinopathy
Combined immunodeficiency
Combined T and B cell dysfunction
Combined features of T cell deficiency and hypogammaglobulinaemia
Severe combined immunodeficiency syndromes
Failure to thrive in children
Opportunistic infection
Overwhelming sepsis
Wiskott-Aldrich syndrome
Thrombocytopenia
Eczema
Infection with encapsulated bacteria
Ataxia telangiectasia
Sinopulmonary disease
Cerebellar ataxia
Oculocutaneous telangiectasia
DiGeorge syndrome
Hypocalcaemia
Recurrent infection
Cardiac disease
Abnormal facial features
Hyper IgM syndromes
Recurrent pyogenic infection
Opportunistic infection
Natural killer cell dysfunction
Recurrent herpes virus infection
Recurrent papillomavirus infection (warts)
Phagocyte defects
Recurrent pyogenic infections
Recurrent abscesses
Ig immunoglobulin
Table 2 Investigations for suspected immunodeficiency
Suspected immunodeficiency
Screening
tests
Advanced investigations
Humoral
immunodeficiency
IgG, IgA, IgM
Full blood count and differential*
Lymphocyte subsets*
IgG subclasses
Specific antibody titres and response to vaccination
Cellular
immunodeficiency
Full blood count and differential*
Lymphocyte subsets*
HIV testing if indicated
IgG, IgA, IgM
Delayed-type hypersensitivity skin tests
Lymphocyte proliferation assays
Natural killer cell cytotoxicity

Ig immunoglobulin
* defer testing until resolution of acute infective illness

Humoral immunodeficiency

Antibody deficiency, or hypogammaglobulinaemia, can occur as a result of intrinsic defects of humoral immunity (primary), or secondary to another pathological condition. It is the most common manifestation of primary immunodeficiency and encompasses a broad range of clinical diagnoses. Clinical presentation can range from asymptomatic, to recurrent, atypical or life-threatening infections. Encapsulated bacteria, such as Streptococcus pneumoniae, Neisseriae species and Haemophilus influenzae, pose a particular threat as well as other bacterial species including Staphylococcus aureus, Pseudomonas aeruginosa, Campylobacter fetus and Mycoplasmaspecies. Recurrent or unusually severe sinopulmonary infection, other infections (gastrointestinal, skin, joint or central nervous system), or evidence of end-organ damage such as bronchiectasis, should alert the doctor to the possibility of an underlying humoral immunodeficiency.

Measuring humoral immunity

The simplest initial investigation for this condition is to quantify immunoglobulin (Ig) concentrations (IgG, IgA and IgM). Normal levels, however, do not exclude a humoral defect and if clinical suspicion is high, then more advanced investigations can be undertaken. This includes measuring antibodies to specific antigens following vaccination to assess if the patient produces a functional antibody response. This is usually performed in conjunction with assessment by a clinical immunologist. Immunoglobulin G subclasses can also be quantified – however the clinical utility of this investigation is somewhat controversial.

Cell-mediated immunodeficiency

Defective T cell-mediated immunity predisposes patients to a broader range of infections than humoral immunodeficiency, including intracellular pathogens, persistent superficial candidiasis or recurrent viral, fungal or protozoal infections (Table 1). Defects can again be classified as either primary, or secondary to extrinsic factors. HIV infection resulting in progressive depletion of CD4T cells is a particular consideration. As helper T cells are required for B cell-mediated antibody production, T cell immunodeficiency can result in functional B cell defects, thus patients with cell-mediated immunodeficiency often have an accompanying hypogammaglobulinaemia. This is termed combined immunodeficiency.

Measuring cellular immunity

Measurement of cell-mediated immunity can be undertaken by both in vitro and in vivo methods. It is, however, more problematic than humoral assessment as assays are plagued by difficulties in standardisation, biological variability, imprecision and technical complexity. Most tests are highly specialised and referral to a clinical immunologist is recommended.

Flow cytometry

The first step in the evaluation of cell-mediated immunity is to quantify circulating immune cells and their subsets by flow cytometric analysis. Patients' blood cells are incubated with fluorochrome-labelled monoclonal antibodies directed against cell surface molecules and analysed by a flow cytometer, which measures light scatter and fluorescence emission from individual cells. Different cell populations (B cells, and CD4/CD8T cells and natural killer cells) can be distinguished based on their scatter profile and surface molecule expression. Absolute cell numbers are calculated as a percentage of the total white cell count and results are compared to age-matched reference ranges. It is important to note, however, that analogous to immunoglobulin measurement, quantification of lymphocyte numbers does not give an indication of their functional capacity. Lymphocyte subset analysis aids in the diagnosis and classification of paediatric severe combined immunodeficiency syndromes, and is also recommended in the evaluation of hypogammaglobulinaemia in common variable immunodeficiency. Quantifying CD4T lymphocytes provides prognostic information and gives an indication of susceptibility to opportunistic infections in patients with HIV infection.

Delayed-type hypersensitivity skin testing

Delayed-type hypersensitivity skin testing provides a functional in vivo assessment of cellular immunity. The skin response following intradermal inoculation of antigen is dependent on antigen-specific memory T cells and results in local inflammation after 48–72 hours due to the recruitment of mononuclear cells (lymphocytes, monocytes) and neutrophils. By convention, a diameter of 5 mm induration is accepted as a positive result. The most widespread use of this type of test is the Mantoux test, which assesses previous exposure to Mycobacterium tuberculosis or Bacillus Calmette-Gué rin (BCG) vaccination by evaluating the skin response to intradermal tuberculin. Other ubiquitous antigens that can be tested include tetanus, candida and certain bacterial antigens. Skin responses are dependent upon previous exposure to the antigen and thus this test is of little use in infants less than six months of age.

Skin testing identifies functional memory T cells to a particular antigen, or the presence of cutaneous anergy. The latter is defined as an impaired cutaneous hypersensitivity response to a panel of common antigens and is consistent with cellular immune dysfunction. Causes of cutaneous anergy are listed in Table 3.

Table 3 Causes of cutaneous anergy*
Cause
Examples
Drugs
Corticosteroids (usually high dose)
Immunosuppressants
Chemotherapy
Immunodeficiency
Ataxia telangiectasia
Severe combined immunodeficiency syndrome
DiGeorge syndrome
Wiskott-Aldrich syndrome
Infection
HIV
Influenza
Measles
Mumps
Active tuberculosis
Other conditions
Malignancy
Chronic lymphocytic leukaemia
Sarcoidosis
Chronic renal failure
Chronic liver disease
Testing errors
Poor technique
Inadequate antigen dose

* impaired skin response to antigen

Lymphocyte proliferation assays

Lymphocyte proliferation assays are indicated if there is a suspicion of a defective cellular immune response either globally or to a specific antigen such as candida. The patient's peripheral blood mononuclear cells are incubatedin vitrofor 3–5 days with either a mitogen (substance which induces cellular division) or a recall antigen (to which the patient has been previously exposed). Radioactive thymidine is added to the culture and subsequently incorporated into the DNA of dividing cells. Radioactivity of the cell culture is measured after 24 hours and is directly proportional to the degree of induced cellular proliferation. Peripheral blood mononuclear cells from a healthy control are evaluated in parallel for comparison.

These assays are technically complex and are only performed by specialist laboratories. As the investigation can be time-consuming, it is advisable to first discuss the appropriateness of testing and choice of assay with the laboratory. Results are affected by immunosuppressive drugs, severe nutritional deficiencies and intercurrent illness1 and these factors must be considered when interpreting results. As with skin testing, the patient must have been previously exposed to the antigen, thus antigen proliferation assays are not feasible in babies lessthan six months of age. Response to mitogens, however, can be performed at any age from birth onwards.2

Other assays measuring lymphocyte activation

Other functional in vitro measures of lymphocyte activation include determining changes in surface marker expression (CD25, CD69, CD71) following activation3 or measurement of intracellular cytokines of T lymphocytes.

T cell proliferation following stimulation can be measured by succinimidyl ester of carboxyfluorescein diacetate (CFSE) dilution techniques,4 or T cell cytokine production quantified by ELISPOT assays. These assays, however, are not in routine use and are confined to research or specialised reference immunology laboratories.

The recently introduced interferon gamma (IFNγ) release assays measure T lymphocyte production of IFNγ in response to antigen exposure thereby providing an assessment of cell-mediated immunity. As with delayed-type hypersensitivity skin testing, clinical application is currently confined to the domain of tuberculosis latency and exposure.

Natural killer cell cytotoxicity assays

Assessing natural killer cells is indicated in patients suffering recurrent infection with herpes virus, or papillomavirus (associated with cutaneous warts). Natural killer cell cytotoxicity is assessed by a 51Cr-release assay in which patients' natural killer cells are incubated with 51Cr-labelled target cells. Lysis of the target cells by natural killer cells leads to the release of radioactivity which can be measured. Natural killer cell dysfunction may occur in patients with CD16 genetic mutations, chronic mucocutaneous candidiasis, severe combined immunodeficiency and other cellular immunodeficiency syndromes.5 These conditions need to be considered and excluded if natural killer cell dysfunction is confirmed. As with T and B lymphocytes, functional natural killer cell deficits can occur even when natural killer cell counts are normal. Natural killer cell assays are technically complex and are rarely performed in diagnostic immunology laboratories.

Conclusion

The evaluation of suspected immunodeficiency is guided by clinical presentation. Screening tests of humoral and cellular immune function are initially performed, followed by referral to a specialist for more advanced investigations if clinically indicated (Table 2). Secondary causes of immunodeficiency, including HIV infection, need to be considered and excluded. When interpreting results, confounding factors such as immunosuppressive drug therapy and patient comorbidities, as well as analytical variables such as assay precision and reproducibility, need to be considered.

Conflict of interest: none declared

Self-test questions

The following statements are either true or false.

1. Persistent superficial candidiasis may be a sign of T cell dysfunction.

2. Normal immunoglobulin concentrations exclude a humoral immunodeficiency.

Answers to self-test questions

1. True

2. False

References

  1. Bonilla FA. Interpretation of lymphocyte proliferation tests. Ann Allergy Asthma Immunol 2008;101:101-4.
  2. Hicks MJ, Jones JF, Thies AC, Weigle KA, Minnich LL. Age-related changes in mitogen-induced lymphocyte function from birth to old age. Am J Clin Pathol 1983;80:159-63.
  3. Caruso A, Licenziati S, Corulli M, Canaris AD, De Francesco MA, Fiorentini S, et al. Flow cytometric analysis of activation markers on stimulated T cells and their correlation with cell proliferation. Cytometry 1997;27:71-6.
  4. Fulcher DA, Wong SWJ. Carboxyfluorescein succinimidyl ester-based proliferative assays for assessment of T cell function in the diagnostic laboratory. Immunol Cell Biol 1999;77:559-64.
  5. Bonilla FA, Bernstein IL, Khan DA, Ballas ZK, Chinen J, Frank MM, et al. Practice parameter for the diagnosis and management of primary immunodeficiency. Ann Allergy Asthma Immunol 2005;94:S1-63.